G8600-03H
G-Protein Coupled Bile Acid Receptor 1 (GPBAR1, M-BAR, BG37, TGR-5)
Size
£832.00
- SKU:
- G8600-03H
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1, Met1-Asn330
- Clone:
- 7H236
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- G protein coupled bile acid receptor 1 (GPBAR1), also called M-BAR, BG37 or TGR-5, is a G-protein coupled receptor superfamily member that is a widely expressed cell surface receptor for bile acids. On macrophages, bile acid engagement of GPBAR-1 suppresses phagocytosis and other functions. Extracellular portions of human GPBAR-1 show ~75% amino acid identity with corresponding regions of mouse GPBAR-1. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells in HEK293 human embryonic kidney cell line Optimal dilutions to be determined by the researcher. Positive Control: THP-1 cells Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice