G-Protein, i alpha 2, (Gia2)

Size

£429.00

SKU:: G8599-07A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: E. coli-expressed human Gai2 (aa 231-351)
Format:: Supplied as a lyophilized powder in 0.09% sodium azide. Reconstitute with 100ul sterile PBS.
Extra Details:: Gai2 is a 40--41kD alpha-subunit of heterotrimeric protein, that shares sequence homology with Gai3 and Gai1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gi2 is expressed widely in tissues and cell lines. Activation of PLC-B by Gai-coupled receptors is the result of release of GBy subunits from Gai. Gai2 regulates potassium channel activation. Mutation at Arg 179 has been identified in tumors. This and the Q205L mutant inhibit adenyl cyclase. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.