G8599-01A
G-Protein, alpha, (Goa)
Size
£869.00
- SKU:
- G8599-01A
- Application:
- WB
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine brain Goa purified by the method of Sternweis and Robishaw.
- Clone:
- 2Q484 (2A)
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of a-subunits (ao1 and a2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C-terminal third of the deduced amino acid sequence. Some of the literature suggests that Transducin is the generic name for the heterotrimeric G protein or possibly Gt. Transducin is composed of several subunits. G proteins are further categorized into different classes, including Go and Gi, based on their aa sequence. Goa is a subunit of Go. Goa could be one subgroup of Transducin. However, there is some literature stating that Go, Gi, and Transducin are all separate groups of G proteins. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:3000. Detects a single protein band of 39-42kD in bovine and rat membranes. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice