G8599-01A-ML650
G-Protein, alpha, (Goa) (MaxLight 650)
Size
£1,132.00
- SKU:
- G8599-01A-ML650
- Application:
- WB
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine brain Goa purified by the method of Sternweis and Robishaw.
- Clone:
- 2Q484 (2A)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of a-subunits (ao1 and a2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C-terminal third of the deduced amino acid sequence. Some of the literature suggests that Transducin is the generic name for the heterotrimeric G protein or possibly Gt. Transducin is composed of several subunits. G proteins are further categorized into different classes, including Go and Gi, based on their aa sequence. Goa is a subunit of Go. Goa could be one subgroup of Transducin. However, there is some literature stating that Go, Gi, and Transducin are all separate groups of G proteins. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:3000. Detects a single protein band of 39-42kD in bovine and rat membranes. Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice