G8586-75B

GPR73A (Prokineticin Receptor 1)

Size

£812.00

SKU:: G8586-75B
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: NSO cells transfected with human GPR73A (Met1-Lys393)
Clone:: 8H181
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: GPR73A is a 7-transmembrane glycoprotein of the GPCR family. The extracellular portions of human GPR73A share 81% and 78% aa identity with corresponding portions of mouse GPR73A and human GPR73B, with non-identity mainly in the N-terminal sequences. Both GPR73A and GPR73B mediate the effects of prokineticins 1 and 2. This includes mitogenic regulation of angiogenesis in endocrine glands and stimulation of contraction in gastrointestinal smooth muscle. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/ml labeled 10e6 human blood-derived monocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.