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G8256X

GnRH Receptor (LH-RH Receptor) (BSA & Azide Free)

Size

£1,017.00

SKU:
G8256X
Purity:
Purified by Protein G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Synthetic peptide corresponding to aa1-29, MANSASPEQNQNHCSAINNSIPLMQGNLPY, from the N-terminal of the human GnRH receptor. MW of Antigen: 54-60kD. Cellular Localization: Cell membrane and cytoplasmic.
Clone:
3F193
Format:
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See G8256.
Extra Details:
GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:100 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Pituitary or normal ovary Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice