G8245
GMP-1 (Gap Modifying Protein 1, SUMO-1)
Size
£761.00
- SKU:
- G8245
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full-length recombinant protein corresponding to human GMP1.
- Clone:
- 6A92
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Transport of macromolecules between the nucleus and cytoplasm is mediated by nuclear pore complexes (NPCs) that are large supermolecular structures that span the nuclear envelope. The Ran protein is required for the bi-directional transport of proteins and RNPs across the NPC. Ran is thought to operate in much the same fashion as other Ras-like GTPases acting as a molecular switch by cycling between the GTP bound and GDP bound forms. Studies have demonstrated that RanGAP1 is highly concentrated at the cytoplasmic periphery of the NPC where it associates with RanBP2. This interaction requires the RanGap1 protein to be conjugated to a ~17kD ubiquitin-like protein termed GMP1(Gap modifying protein 1) or SUMO-1(small ubiquitin-related modifier). The unmodified 70kD form of RanGAP1 is exclusively cytoplasmic, whereas the 90kD modified form of RanGap1 associates with the cytoplasmic fibers of the NPC. Therefore, the ubiquitin-like GMP1(SUMO-1) protein functions in a novel way by modulating the partitioning of RanGAP1 between the cytoplasm and NPC. GMP1 is a small polypeptide which shares homology with ubiquitin and appears to be involved in protein targeting. In addition to RanGAP-1, at least three other intranuclear proteins are thought to be modified with GMP1. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilution: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunofluorescence: 10-20ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: Rat liver nuclear envelopes, total lysates derived from NIH 3T3 and HeLa cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice