G8245-ML650
GMP-1 (Gap Modifying Protein 1, SUMO-1) (MaxLight 650)
Size
£1,017.00
- SKU:
- G8245-ML650
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full-length recombinant protein corresponding to human GMP1.
- Clone:
- 6A92
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Transport of macromolecules between the nucleus and cytoplasm occurs bi-directionally and is mediated by nuclear pore complexes (NPCs). NPCs are large supermolecular structures that span the nuclear envelope. Although ions, metabolites, and small macromolecules can passively diffuse through the NPC, most larger macromolecules such as proteins and ribonucleoproteins (RNPs) must be transported across the NPC by specific signal- and energy -dependent mechanisms. The Ran protein is required for the bi-directional transport of proteins and RNPs across the NPC. Ran is thought to operate in much the same fashion as other Ras-like GTPases acting as a molecular switch by cycling between the GTP bound and GDP bound forms. Nucleotide exchange by Ran is catalyzed by the GTP exchange factor RCC1, while GTP hydrolysis is catalyzed by the GTPase-activating protein RanGAP1. Two other proteins which bind Ran-GTP and possibly affect GTP hydrolysis include RanBP1 and RanBP2 (Nup 358). The mammalian RanGap-1 protein was originally purified from HeLa cells as a 65-70kD homodimer. Subsequent studies have demonstrated that RanGAP1 is highly concentrated at the cytoplasmic periphery of the NPC where it associates with RanBP2. This interaction requires the RanGap1 protein to be conjugated to a ~17kD ubiquitin-like protein termed GMP1 (Gap modifying protein 1) or SUMO-1 (small ubiquitin-related modifier). The unmodified 70kD form of RanGAP1 is exclusively cytoplasmic, whereas the 90-kD modified form of RanGap1 associates with the cytoplasmic fibers of the NPC. Therefore, the ubiquitin-like GMP1 (SUMO-1) protein functions in a novel way by modulating the partitioning of RanGAP1 between the cytoplasm and NPC. As described above, GMP1 (SUMO-1) is a small polypeptide which shares homology with ubiquitin and appears to be involved in protein targeting. Applications: Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Immunofluorescence: 10-20ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: Rat liver nuclear envelopes, total lysates derived from NIH 3T3 and HeLa cells. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice