G8235-58
Glypican 6 (Glypiated Proteoglycan 6)
Size
£825.00
- SKU:
- G8235-58
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp24-Val527 from human Glypican 6 expressed in NS0 cells.
- Clone:
- 7H114
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Glypican 6 (glypiated proteoglycan 6) belongs to the family of membrane heparin sulfate proteoglycans that are linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. The glucosaminoglycans (GAGs) attachment sites of glypican family proteins are localized close to the cell membrane in the C-terminal region. Glypicans are thought to function as low-affinity binding proteins that retain signaling molecules (cytokines, growth factors, proteases) at the cell surface. Mature human and mouse glypican share 98% amino acid sequence identity. Glypican 6 is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Applications: Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry (Intracellular): 0.25ug/10e6 cells using HepG2 human hepatocellular carcinoma cell line Western Blot: 1ug/ml using recombinant human Glypican 6 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice