G8235-50
Glypican 3 (Glypiated Proteoglycan 3) (APC)
Size
£832.00
- SKU:
- G8235-50
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln25-Val558 from human Glypican 3, expressed in NS0 cells.
- Clone:
- 7H23
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Glypicans are GPI-anchored heparan sulfate proteoglycans that include a large globular cysteine-rich domain with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Glypicans are thought to function as low-affinity binding proteins that retain signaling molecules (cytokines, growth factors, proteases) at the cell surface. Glypicans are widely expressed in adult and fetal tissues. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. Using HepG2 human hepatocellular carcinoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice