Glypican 1 (Glypiated Proteoglycan 1)

Size

£882.00

SKU:: G8235-40
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Asp24-Ser530 from human Glypican 1, expressed in NSO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Glypican 1 (glypiated proteoglycan 1) is a 200kD, highly glycosylated member of the GPI-linked Glypican family of proteins. It is expressed by both epithelial and mesodermally-derived cells, and appears to use its proteoglycan adduct to regulate growth factor signaling and availability. Human Glypican 1 is synthesized as a 535 amino acid (aa) proprecursor that contains a 507 mature region (aa 24-530) and a 28 aa C-terminal prosegment. There are three potential splice variants. One shows an alternate start site at Met73, a second incorporates the same start site with a 65 aa substitution for aa 295-558, and a third shows a deletion of aa 184-558. Mature human Glypican 1 is 91% aa identical to mouse Glypican 1. Applications: Suitable for use in Direct ELISA, Immunocytochemistry, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells validated using MDA-MB-231 human breast cancer cell line Western Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/ml using immersion fixed MDA-MB-231 human breast cancer cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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