G8170-50K
Glycogen Synthase Kinase 3 beta (GSK3b)
Size
£761.00
- SKU:
- G8170-50K
- Application:
- ICC
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- E. coli-derived rhGSK3b
- Clone:
- 8H132
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3α and GSK-3β) share 85% amino acid identity. GSK-3β, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human Glycogen Synthase Kinase-3 beta (rhGSK-3β; aa 1-420; Accession # P49841). Applications: Immunocytochemistry Recommended Dilution: Immunocytochemistry: This antibody was used at a concentration of 25ug/mL with the appropriate secondary reagents to detect GSK-3β in fixed human MCF-7 and HeLa cells. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice