G8170-47-HRP
Glycogen Synthase Kinase 3, phosphorylated (Y279/Y216) (GSK3) (HRP) (Drosophila)
Size
£1,081.00
- SKU:
- G8170-47-HRP
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide (aa203-219, C-KQLLHGEPNVS[pY]ICSRY) corresponding to the catalytic domain (between subdomain VII & VIII) of Drosophila GSK-3/shaggy enzyme coupled to ovalbumin (Accession# NM_057366).
- Clone:
- 0.T.57
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. The enzyme is the mammalian homolog of the zeste-white3 (shaggy) homeotic gene of Drosophila melanogaster and has been implicated in the regulation of the c-Jun/AP-1 transcription factor. Genetic and biochemical evidence now indicates that GSK-3 plays a role in intracellular signaling pathways. For example, during insulin and growth factor signaling the GSK-3 beta isoform becomes phosphorylated on its N-terminal serine-9 residue, presumably by either the action of MAPKAP Kinase-1 (p90 S6 kinase, Rsk-2) or p70 S6 kinase. Such phosphorylation of GSK-3 blocks its ability to phosphorylate c-Jun, protein phosphatase inhibitor 2 or the GSK-3 substrate peptide. GSK-3 is constitutively phosphorylated at this tyrosine residue in resting cells. This immunogen is identical in 15 of 17 residues in human and rat GSK-3a (Y279) and GSK-3b (Y216). The phosphotyrosine residue can be removed from GSK-3 by treatment with PTP1B. Applications: Suitable for use in Western Blot. Not suitable for use in Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects phospho-GSK-3 in RIPA lysates from human Jurkat cells. Previous lots detected phospho-GSK-3 in mouse 3T3/A31, human A431 and rat L6 cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8170-47. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: J9000: Jurkat lysate. Use 20ug per lane for minigels. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice