G8170-44F
Glycogen Synthase Kinase 3 beta (GSK3b)
Size
£377.00
- SKU:
- G8170-44F
- Purity:
- Purified by Protein A affinity chromatography from serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa335-349, CAHSFFDELRDPNVK from rat GSK3b, conjugated to KLH.
- Format:
- Supplied as a liquid PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- GSK3 beta, also known as Tpk1 (tau protein kinase-1), is a 47kD protein-serine/threonine kinase with broad specificity that phosphorylates and inactivates glycogen synthase, the phosphatase 1 inhibitor protein, and other proteins that feature a (S/T)PXX(Tp/Sp) recognition motif. It is ubiquitously distributed, but highly expressed in mammalian brain, and occurs in two isoforms, 51kD (alpha) and 47kD (beta). This monomeric proline-directed kinase is inhibited when it is phosphorylated by protein kinase B and S6 protein kinases. It is activated when it is phosphorylated by an unknown protein-tyrosine kinase. GSK3beta is inhibited in response to insulin. GSK3beta is phosphorylated and inactivated by protein kinase C, p70, S6 kinase and Rsk-1. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (Colorimetric): 1:1000 Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by researcher. Positive Controls: HeLa Cell Lysate, Mouse Brain Tissue Extract Rat Brain Tissue Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice