G8170-43

Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (GSK3b)

Size

£812.00

SKU:: G8170-43
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa6-12 (RTT[pS]FAE) of human GSK3b (KLH). The immunizing sequence is identical in mouse, rat and Xenopus.
Clone:: 2D3
Format:: Supplied as a liquid in PBS, PEG, sucrose, 0.09% sodium azide, 50% glycerol.
Extra Details:: Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. The enzyme is the mammalian homolog of the zeste-white3 (shaggy) homeotic gene of Drosophila melanogaster and has been implicated in the regulation of the c-Jun/AP-1 transcription factor. In mammals, this protein serine kinase is encoded by two related genes called GSK-3a (51kD) and b (46kD). Genetic and biochemical evidence now indicates that GSK-3 plays a role in intracellular signaling pathways. For example, during insulin and growth factor signaling the GSK-3 beta isoform becomes phosphorylated on its N-terminal serine-9 residue, presumably by either the action of MAPKAP Kinase-1 (p90 S6 kinase, Rsk-2) or p70 S6 kinase. Such phosphorylation of GSK-3 blocks its ability to phosphorylate c-Jun, protein phosphatase inhibitor 2 or the GSK-3 substrate peptide. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects phosphorylated GSK3b (pSer9) in RIPA lysates from Hep G2 cells stimulated with 100uM pervandate and also from rat L6 cells stimulated with 15nM IGF-1.Non-stimulated HUVAC cell lysate and pervanadate-stimulated HUVAC cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with G8170-43 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice