G8170-40D3

Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a)

Size

£812.00

SKU:: G8170-40D3
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Phosphopeptide containing human, mouse, and rat GSK-3 alpha S21 site
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 alpha is inhibited by phosphorylation at S21 by Akt and other kinases. Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer’s disease, bipolar disorder, and cancer. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human thymus Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.