G8170-30
Glycogen Synthase, phosphorylated (Ser641)
Size
£639.00
- SKU:
- G8170-30
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide, corresponding to residues surrounding Ser641 of human liver glycogen synthase.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Glycogen synthase (GS) is the key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS, the muscle isoenzyme and the liver form (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of allosteric activator glucose-6-phosphate, which converts the enzyme into a better substrate for phosphatase and induces its dephosphorylation and activation (4). Ser641 is one of the most important phosphorylation sites for regulation of glycogen synthase (5). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice