G8170-18D
Glycogen Synthase
Size
£626.00
- SKU:
- G8170-18D
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the sequence of human glycogen synthase.
- Clone:
- 7H22(15B1)
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Extra Details:
- Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle isoenzyme and the liver form. Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific. GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:25 Immunohistochemistry (Paraffin): 1:100. Use citrate as unmasking buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice