G8153-40B
GKN1 (Gastrokine-1, 18kD Antrum Mucosa Protein, AMP-18, Protein CA11, AMP18, CA11, Gastrokine 1, UNQ489/PRO1005)
Size
£832.00
- SKU:
- G8153-40B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to aa35-199 from human Gastrokine 1 expressed in E. Coli (Q9NS71). Species sequence homology: Recombinant mouse Gastrokine 1 (3%).
- Format:
- Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
- Extra Details:
- Gastrokine 1 is an 18kD member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165aa in length. Based on the SwissProt sequence, it possesses one BRICHOS domain aa54-150 that contains a mitogenic sequence aa97-117. There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa21-185 (aa35-199 of SwissProt Q9NS71), human Gastrokine 1 shares 65aa identity with mouse Gastrokine 1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice