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G8135-28D

Glutathione S-Transferase Yb1, Rat (GST)

Size

£492.00

SKU:
G8135-28D
Purity:
Homogeneous by SDS gel electrophoresis.
Storage Conditions:
-20°C
Supplier:
United States Biological
Format:
Suspended in 20mM Tris buffer (pH 8.5).
Extra Details:
Source: Rat Liver Application: Suitable for use as a standard for Western blotting or for ELISAs. Other applications not tested. Optimum dilutions to be determined by researcher. Specific activity: 21.5units/mg Definition of unit: One unit is the amount of enzyme that catalyzes the conjugation of 1uM of 1-chloro-2,4-dinitrobenzene (CDNB) to glutathione in one minute at room temperature in 100mM potassium phosphate buffer, pH 6.5. [J.B.C., 249 : 7130-7139 (1974)] Packaging: Each vial contains sufficient enzyme for up to 10 assays using CDNB as substrate in a spectrophotometric assay. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice