G8135-25J
Glutathione S-Transferase, pi (GSTP1, GSTpi1, GST3, DFN7)
Size
£512.00
- SKU:
- G8135-25J
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the C-terminus of human GSTpi which is different from the related mouse sequence by two sequences.
- Format:
- Supplied as a lyophilized powder from PBS, 50% glycerol. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11. Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry (Formalin/paraffin): 1-2ug/ml. Boiling the sections is required Immunocytochemistry (Acetone fixed cells): 0.5-1ug/ml. Antigen retrieval by Pepsin and Trypsin is required. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice