Glutathione Transferase-Alpha (GST-alpha, GST A1-1, Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1) (Not for Export EU)

Size

£704.00

SKU:: G8135-10A
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human GST-alpha/GST A1-1 fusion protein with N-terminal His-tag (25kD), expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y. GSTA1: The alpha gene cluster consists of 5 genes GSTA1-GSTA5; the GSTA1 contains 222aa (chr 6p12), widely expressed in human tissues of liver, kidney, testis, adrenal and pancrease. GST alpha 1- 1 concentration as a marker for hepatocellular impairment, GSTA1 concentration in blood is a more specific marker than AST or ALT, and has a shorter half-life than ALT The GSTA1 shows enzyme activity towards CDNR; NBD-CI; delta 5 AD, PGE2 AND PGF2a synthase. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000 Western Blot: 1:500-1:3000 (chemiluminescent) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice