G8135-04F-ML650
Glutathione S-Transferase, Schistosoma japonicum (GST) (MaxLight 650)
Size
£856.00
- SKU:
- G8135-04F-ML650
- Purity:
- Purified from mouse ascites by Protein A chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST protein from Schistosoma japonicum expressed from the pGEX vector
- Clone:
- GST 3-4C
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase. Using this fusion system, a full-length protein or a shorter polypeptide sequence is linked to the C terminus of the 26kD GST protein from Schistosoma japonicum. The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads. The fusion protein is subsequently eluted from the glutathione agarose in the presence of free glutathione. Antibodies to GST can be utilized as reagents for detecting the pGEX expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins. Applications: Suitable for use in FLISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1.0ug/ml Western Blot: 1ug/ml Immunoprecipitation: 2-5ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice