G8135-02R
Glutathione S-Transferase (GST)
Size
£484.00
- SKU:
- G8135-02R
- Purity:
- Purified from mouse ascitic fluids by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant Glutathione S transferase (GST) purified from E. coli
- Clone:
- 1E5
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% Thimerosal.
- Extra Details:
- Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors Hybridoma: Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant GST protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice