G8135-01D

Glutathione S-Transferase A1-1, Recombinant Human (GST)

Size

£806.00

SKU:: G8135-01D
Molecular Weight:: 23
Purity:: ≥95% by Coomassie stained SDS gel.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM DTT, 5mM EDTA, 50% glycerol.
Extra Details:: Human recombinant protein produced from overexpressing plasmid in E. coli Specific Activity: As reported. Determined using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1mM) in 100mM phosphate buffer, pH 6.5 at RT. Note: This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. These considerations should provide guidance when assaying this product under low concentrations or when using substrates other than CDNB. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.