Glutathione

Size

£393.00

SKU:: G8123-15
Purity:: Purified.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Glutathione-glutaraldehyde.
Format:: Supplied as a liquid in 10mM PBS, 1% sterile goat serum. No preservative added.
Extra Details:: Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Prepare enough G8125-15 for the day’s use by diluting 100X with 1% GSPBT. Using silver-intensified immunogold or fluorescence. Samples should be fixed with 0.2%–2.5% glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative). The minimum glutaraldehyde concentration is 0.02%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: We do not recommend adding glycerol as a cryopreservative.