G7210-50
Glutaredoxin-5, Recombinant, Human (Glutaredoxin 5, GLRX5, GRX5, C14orf87, FLB4739, Glutaredoxin-related Protein 5 Mitochondrial, Monothiol Glutaredoxin-5, PR01238, PRO1238, MGC14129)
Size
£453.00
- SKU:
- G7210-50
- Application:
- WB
- Molecular Weight:
- 18.8
- Purity:
- ~ 85% (SDS-PAGE). Purified by using conventional chromatography techniques.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris HCl, pH 8.0, 0.1M NaCl, 20% glycerol.
- Extra Details:
- GLRX5 is a small redox enzyme of approximately one hundred amino-acid residues which uses glutathione as a cofactor. This protein is oxidized by substrates, and reduced non-enzymatically by glutathione. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Recombinant human GLRX5 protein, fused to His-tag at N-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-157 of human GLRX5 6x His tagged expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice