G7116-05
Glutamic Acid Decarboxylase (GAD)
Size
£953.00
- SKU:
- G7116-05
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified rat brain glutamic acid decarboxylase (GAD).
- Clone:
- 2Q61 (GAD-6)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- The enzyme glutamic acid decarboxylase (GAD) catalyzes the synthesis of g-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. GAD is the rate-limiting enzyme in the biosynthesis of GABA from L-glutamic acid, and it is used extensively as a marker for GABAergic neurons and synaptic terminals. GAD has been observed in pancreatic islet cells and identified as an autoantigen associated with the development of insulin-dependent diabetes mellitus. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: ≤1ug/ml Optimal dilutions to be determined by the researcher. Controls: Rat brain tissue Human brain lysate Immunohistochemistry Protocol: 1. Perfuse rats with 100mM phosphate buffer, pH 7.4, containing 1% paraformaldehyde, 0.34% L-lysine and 0.05% m-periodate (1% PLP). 2. Postfix brains in 1% PLP for 1-2 hours. 3. Transfer brains to 100mM phosphate buffer containing 30% sucrose and gently agitate on a shaker platform at 4°C for 48-60 hours. 4. Using a sliding microtome, cut 30um sections of frozen cerebellum. As the sections are cut, collect them in a vial of cold 100mM phosphate buffer. 5. Incubate sections in PBS containing 1.5% normal serum and 0.2% Triton X-100 for 30 minutes. 6. On a shaker platform, incubate sections with G7116-05 (diluted 1ug/ml in PBS containing 1.5% normal serum and 0.2% Triton X-100) for 12-36 hours at 4°C. 7. On a shaker platform, rinse sections eight times, 10-15 minutes per rinse, in PBS. 8. Detect with standard secondary antibody detection system (PAP, ABC, etc.). 9. Mount sections, dehydrate, and apply coverslips. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice