G7116-01

Glutamic Acid Decarboxylase (GAD)

Size

£767.00

SKU:: G7116-01
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 16aa peptide from GAD65/67 of human, conjugated to KLH. Location: ~C-terminus. Species Sequence Homology: Mouse, rat - 100%; Highly conserved in gold fish, chicken, feline, porcine and zebra fish.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: g-Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter. The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.115). In the CNS GAB is entirely restricted to GABAergic neurons. GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus. Cloning of GAD genes have identified two subtypes: GAD65 (65kD; human 585 AA chromosome 10) and GAD67 (67kD; human 594 AA, chromosome 2) share approx. 65% amino acid homology. The N-terminus is the most divergent while the C-terminus is highly conserved. Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5'-phosphate suggesting their activities are differentially regulated. GAD67 is cytosolic, while GAD65 is membrane associated. GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain. Applications: Western Blot: 1-10ug/ml using Chemiluminescence technique. ELISA: 1:10,000-1:100,000; using 50-100ng control peptide/well. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice