G5012-03A

Glutaredoxin 2 (Glutaredoxin-2, Mitochondrial, GLRX2, GRX2, CGI-133)

Size

£453.00

SKU:: G5012-03A
Application:: IP
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human protein purified from E.coli.
Clone:: 10K207
Format:: Supplied as a liquid in PBS, 50% glycerol.
Extra Details:: Glutaredoxin (Grx), also known as thiol transferase, is a small heatstable oxidoreductase. Grxs form part of the glutaredoxin system, comprising NADPH, GSH and glutathione reductase, which transfers electrons from NADPH to glutaredoxins via GSH. First recovered in E.coli as GSH-dependent hydrogen donors for ribonucleotide reductase, Grx catalyzes GSH-disulfide oxido-reductase via two redox-active cysteine residues. The active sequence (Cys-Pro-Tyr-Cys) is conserved in a variety of species. The 12kD dithiol protein has a role in reduction of mixed disulfides in cells exposed to oxidative stress. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-2ul/400ul lysates Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.