G4905-02
Glycoprotein V (CD42d, GPV, Platelet Glycoprotein V, GP5)
Size
£800.00
- SKU:
- G4905-02
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln17-Gly523 (predicted) of human Glycoprotein V, expressed in NS0 cells. Species Sequence Homology: equine; 81%, rat; 71%, mouse; 70%.
- Clone:
- 9L830
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- GPV (platelet Glycoprotein V; also CD42d) is a 83kD member of the leucine-rich (LR) repeat family of proteins. On the platelet surface, it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for von Willebrand factor. Mature human GPV is a 544aa type I transmembrane glycoprotein. In its extracellular domain, it contains 15 LR repeats (aa73-472), and possesses a short 15aa cytoplasmic tail. GPV is naturally cleaved by ADAM10 and ADAM17 after Pro493, generating a 75-80kD soluble fragment. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using human platelets Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice