G4005-50A-ML650
Glutamate Transporter EAAC1 (MaxLight 650)
Size
£873.00
- SKU:
- G4005-50A-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids 161-177 of the rat glutamate transporter EAAC1 sequence.
- Clone:
- 3G157
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters (2) are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene (3) develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months. Applications: Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 1-5ug/ml Immunoprecipitation: 1-5ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice