G4003-91A-BIOTIN
Glutamate/Aspartate Transporter 2 (GLT1, EAAT2, Excitatory Amino Acid Transporter 2) (Biotin)
Size
£1,099.00
- SKU:
- G4003-91A-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Linear peptide corresponding to the C-terminus of human Glutamate/Aspartate Transporter 2. Species Sequence Homology: rat
- Clone:
- 10H26 (G6)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- The activity of Glutamate/Aspartate Transporter 2 (also known as GLT1), the main glutamate transporter in the CNS, can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the Glutamate/Aspartate Transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the Glutamate/Aspartate Transporter 2. Exposure to PMA increases the ubiquitylation of the Glutamate/Aspartate Transporter 2 in transfected cells and in the rat brain, and this ubiquitylated Glutamate/Aspartate Transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Glutamate/Aspartate Transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Mouse brain tissue lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice