Glutamate (Inhibitory Protein Factor, IPF)

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£754.00

SKU:: G4000-71
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic 10 amino acid peptide near the N-terminus of human IPF (KLH coupled).
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, before the addition of glycerol to 40%.
Extra Details:: Inhibitory protein factor (IPF) refers to a three distinct proteins with ~mol wt of 138kDa (IPF-alpha), 135kD (IPF-beta), and 132kD (IPF-gamma). IPF-a is derived from a ubiquitous, non-erythroid brain spectrin called alpha-Fodrin, a well-characterized protein previously implicated in such diverse activities as exocytosis/endocytosis, apoptosis, and NMDA-receptor activation. However, a-Fodrin itself has no effect on glutamate uptake. The N-terminal 1-20 aa of IPF-a, IPF-b, and IPF-g are identical with 26-45 aa of a-Fodrin (mol wt ~240kD). Therefore, it appears that some identified protease(s) may generate IPF-a from a-Fodrin. IPF does not inhibit ATP-independent uptake, norepinephrine uptake into chromaffin vesicles, and Na-dependent glutamate uptake into synaptosomes. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications have not been tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL technique. ELISA: 0.5-1ug/ml. Coat plates with 1ug/ml control peptide. Optimal dilutions to be determined by the researcher. Control Peptide: G4000-72 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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