G4000-20
Glutamate Decarboxylase 67 (GAD67)
Size
£889.00
- SKU:
- G4000-20
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to rat GAD67
- Clone:
- 3H404 (1G10.2)
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide
- Extra Details:
- Glutamic acid decarboxylase (GAD) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% amino acid identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. GAD67 is cytoplasmic (594aa), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation or disease progression. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:10,000; Using ECL on rat and mouse brain lysate. Immunohistochemistry (Paraffin): 1:500; No epitope retrieval necessary. Optimal dilutions to be determined by the researcher. Positive Control: SKNSH cell lysate (human neuroblastoma), mouse cerebral cortex Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice