G4000-12E
Glutamic Acid Decarboxylase (GAD-67)
Size
£819.00
- SKU:
- G4000-12E
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Synthetic peptide corresponding to a region near the C- terminus of Glutamic Acid Decarboxylase (GAD-67) (KLH). Species sequence homology: human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) 100%.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Extra Details:
- Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66.9kD protein (594aa) selectively expressed in gabanergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter b-amino butyric acid (GABA). An affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells Western Blot: 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice