G4000-09D
Glutamate Decarboxylase 65 (Glutamate Decarboxylase 65kD Isoform, GAD65, GAD-65, 65kD Glutamic Acid Decarboxylase, Glutamate Decarboxylase 2, GAD2, MGC161605, MGC161607)
Size
£642.00
- SKU:
- G4000-09D
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-TLEDNEERMSRLSK, from human GAD2, at the internal region
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- GAD65 catalyzes the formation of gamma-aminobutyric acid from L-glutamic acid. It is an autoantigen in type I diabetes and is transiently activated in response to the demand for extra GABA in neurotransmission. It cycles between an active holo form and an inactive apo form. The gene on chromosome 10p12 is a candidate for Human obesity. The cDNA encodes a Mr 65,000 polypeptide, with 585aa residues. The pathogenic role for this enzyme has been identified in the Human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, observed in human brain lysates ~65+70kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice