G3900-80E

GLUT 7 (Glucose Transporter)

Size

£767.00

SKU:: G3900-80E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 12 AA peptide sequence near the C-terminus of rat Glucose Transporter 7 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.
Format:: supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. This antibody identified a major band at about 52kD in rat liver microsomal membranes (2). ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Immuoprecipitation: use at 5-10 ul neat serum per 100ug of rat brain membranes or 1-10ug affinity pure. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.