G3900-80B
GLUT 7 (Glucose Transporter)
Size
£754.00
- SKU:
- G3900-80B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 14 AA peptide sequence near the C-terminus of rat Glucose Transporter 7 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.
- Format:
- Supplied as liquid in 100mM Tris, pH 7.4 and 0.2% BSA, 0.05% sodium azide, 40% glycerol.
- Extra Details:
- Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family). Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, Mol. Wt. 40-60 kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot:1-10ug/ml using ECL. Detects a major band at about 52kD in various tissues (2). ELISA: 0.5-1ug/ml, Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: G3900-80C GLUT 7, Rat Control Peptide. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice