G3900-75K

GLUT 6 (Glucose Transporter)

Size

£754.00

SKU:: G3900-75K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 16aa synthetic peptide sequence near the cytoplasmic, C-terminus of human GLUT 6, coupled to KLH
Format:: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Extra Details:: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. GLUT isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT 6/SLC2A6, originally described as GLUT 9 has been reassigned as GLUT 6. GLUT 6 (human 507aa; ~48kD; chromosome 9q34) is most closely related to GLUT 8 (~45% homology). It is highly expressed in brain, spleen and peripheral leukocytes. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Control peptide (Cat# G3900-75) can be used to coat ELISA plates at 1ug/ml. Western Blot:1-10ug/ml using ECL. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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