G3900-65L

GLUT 5 (Glucose Transporter)

Size

£754.00

SKU:: G3900-65L
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A cytoplasmic 13 aa peptide sequence near the C-terminus of human glucose/fructose transporter (Glut-5). (KLH coupled)
Format:: Supplied as liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Extra Details:: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80 kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662 amino acids; ~75 kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Applications: Suitable for use in ELISA, Western Blot ,Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml (ECL). Rat glut-5 is ~60kD in rat jejunum membranes that is slightly larger that that reported for human glut-5 (50-55kD). As opposed to human tissues, rat glut-5 mRNA has not been detected in rat testis, adipose tissues and skeletal muscle. In rat kidney, a protein of ~42kD was detected. ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control peptide: G3900-65M Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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