G3900-65H
GLUT 5 (Glucose Transporter) (Not for Export EU)
Size
£704.00
- SKU:
- G3900-65H
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A cytoplasmic 12 AA peptide sequence near the C-terminus of human glucose/fructose transporter (Glut-5) (1). A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH
- Format:
- Supplied as a liquid, neat serum, 40% glycerol.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. An antibody raised to the rat C-terminal 13aa of glut-5 has been shown to react with a protein band of about 60kD in the rat jejunum membranes that can be competed with the control peptide (2, 3). The MW. Of Glut 5 is larger than that reported for human Glut 5 (50-55kD) (4). As opposed to human tissues, rat Glut-5 mRNA has not been detected in rat testis, adipose tissues, and skeletal muscle (2, 4). In rat kidney, a protein of about 42kD was detected (6). Differences in MW. Of Glut-3 in various tissues has also been noted. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice