G3900-65D
GLUT 5 (Glucose Transporter)
Size
£390.00
- SKU:
- G3900-65D
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresonding to 13aa near the C-terminal, cytoplasmic domain of rat Glut-5, conjugatd to KLH. Species Sequence Homology: human: 61%, mouse: 53%.
- Format:
- Supplied as liquid in PBS, pH 7.4, 0.1% BSA.
- Extra Details:
- Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-60kD) and Sodium glucose co-trasporter-1 (SGLT-1, 662aa, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml using ECL. The antibody has been shown to react with a protein band of about 60kD in the rat jejunum membranes that is slightly larger than that reported in human GLUT 5 (50-55kD). In rat kidney, a protein of ~42kD was detected. ELISA: 1:1000- 1:10,000. Use G3900-65G control peptide to coat ELISA plates at 1ug/ml. Immunohistochemistry: See reference (2). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice