G3900-50F

GLUT4

Size

£863.00

SKU:: G3900-50F
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partially purified vesicles containing Glut4
Clone:: 10C380 (1F8)
Format:: Supplied as a liquid in PBS.
Extra Details:: Glut4 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut4 is expressed in the insulin-responsive tissues, muscle and adipose tissue. Insulin stimulation leads to translocation of Glut4 from intracellular stores to the cell surface resulting in the enhancement of glucose uptake. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml in immersion fixed L6 rat myoblast cell line (differentiated to muscle) Immunohistochemistry: 5-25ug/ml in immersion fixed paraffin-embedded sections of rat heart tissue Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.