G3900-26
GLUT 3 (Glucose Transporter)
Size
£767.00
- SKU:
- G3900-26
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 12 aa C-terminal peptide sequence near the C-terminus of mouse glucose transporter-3 (Glut-3). A cysteine has been added at the NH2 terminus for coupling to KLH.
- Format:
- Supplied as a liquid in 10mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
- Extra Details:
- Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family). Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, MW 40-60 kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. Major band at ~45kD. ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml (antigen) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Store long-term at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice