G3900-15D

GLUT 2 (Glucose Transporter, Insulin Regulatable)

Size

£754.00

SKU:: G3900-15D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide corresponding to 16aa near the C-terminal, cytoplasmic domain of rat Glut-2 conjugated with KLH. Subcellular Location: multi-pass membrane protein. Species Sequence Homology: mouse and human-93%, ovine-84%.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, 40-80kD) and sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Western Blot: 1-10ug/ml using Chemiluminescence technique. Immunohistochemistry: 2-10ug/ml Optimal dilutions to be determined by the researcher. Recommended Control Peptide: G3900-15F: GLUT 2, Rat (Glucose Transporter, Insulin Regulatable) Recommended Tissues: Liver, intestine, kidney, and beta-pancreatic islet cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice