G3900-15B

GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

Size

£927.00

SKU:: G3900-15B
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Glut-2. Epitope: Extracellular domain.
Clone:: 11C101
Format:: Supplied as a liquid in PBS, pH 7.5. Preservative free.
Extra Details:: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Human Glut-2 (GTR2, 524aa, chromosome 3q26.1-q26.3, ~60kD) belongs to the family of solute carrier family 2, member 2 or Slc2a2 or facilitative glucose transporter. Glut-2 likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. It is a multi-pass membrane protein. Primarily expressed in liver, insulin-producing beta cell, small intestine and kidney. Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS, a rare, autosomal recessive mode and characterized by hepatorenal glycogen accumulation, and impaired utilization of glucose and galactose. Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1). Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/ml (Chemiluminescence). Do not heat the samples prior to loading to prevent gluts aggregation. Immunohistochemistry; 5-20ug/ml in 4% paraformaldehyde, 0.15% picric acid for 20 minutes and then permeabilized with 0.1% Triton X-100. Flow Cytometry: ~10ug/ml (10e6 cells) Perform the antibody binding in small volume of ~200ul. Optimal dilutions to be determined by the researcher. Positive Control: Human Caco-2 cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice