G3900-05K

GLUT 1 (Glucose Transporter 1)

Size

£754.00

SKU:: G3900-05K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide correesponding to 15aa near the C-terminus, cytoplasmic domain of human Glut-1 (KLH). Species Sequence Homology: porcine, bovine, rabbit, mouse and rat; 100%.
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue- specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml using ECL. Identifies a major band at about 42-45kD from purified rat brain membranes. ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: G3900-05L: GLUT 1 (Glucose Transporter 1) Control Peptide Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice