G3500-13G-ML490
GluR2 (Glutamate Receptor) (MaxLight 490)
Size
£1,036.00
- SKU:
- G3500-13G-ML490
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein containing aa175-430 from the N-terminal region of GluR2
- Clone:
- 3G156 (6C4)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. Regulation of fast excitatory synaptic neurotransmission is mediated primarily by ionotropic glutamate receptors (iGluR)-ligand-gated cation (principally Ca2+) channels found on postsynaptic neurons throughout the central nervous system. iGluR receptors participate in neural development, plasticity, learning and memory, and are implicated in excitatory and neurodegenerative pathologies including stroke, Alzheimer’s disease, Huntington’s chorea and amyotrophic lateral sclerosis. Pharmacological properties and amino acid sequence similarities allow classification of iGluR into three main subfamilies named for their principal agonists: 1. NMDA receptors and the two non-NMDA receptor subfamilies 2. AMPA receptors and 3. kainate. Glutamate signaling also occurs via a family of G-protein coupled glutamate receptors referred to as the metabotropic glutamate receptors (mGluR). mGluRs can modulate excitatory synaptic transmission by regulating neurotransmitter release, influencing ion channel activity, and altering synaptic plasticity. Both ionotropic and metabotropic glutamate receptors are also present on macroglia (astrocytes and oligodendrocytes) where they have been shown to influence gene expression and may be involved in precursor cell proliferation and differentiation. Applications: Suitable for use in FLISA, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice