G3057-43-ML650

Glucose Regulated Protein 94 (Grp94, gp96) (MaxLight 650)

Size

£971.00

SKU:: G3057-43-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the N-terminal region of the mouse and human gp96 protein. Species Sequence Homology: porcine, bovine, and canine; 100%
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The tumor rejection antigen (gp96), also known as glucose-regulated protein 94 (GRP94), is a member of the heat shock protein (HSP) family. The gp96 protein is up-regulated by stress conditions such as glucose starvation and heat shock, and its function is regulated through a ligand-dependent conversion from an active to an inactive conformation. gp96 is involved in both innate and adaptive immunity. A study has shown that a B-cell line deficient in gp96 is unresponsive to microbial stimuli because toll-like receptors (TLRs) are retained intracellularly. However, normal surface levels of TLRs were restored when these cells were transfected with a cDNA encoding gp96. The tumor rejection antigen can activate the release of nitric oxide by antigen presenting cells (APCs) and it also has a relevant role in the regulation of immune responses to pathological cell death. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 1-3ug/ml. Identifies a single band at ~96kD Immunoprecipitation: 1-5ug/ml Immunohistochemistry: 0.5-1ug/ml Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining for formalin-fixed, paraffin-embedded (FFPE) tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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